Premium
Equivalent stimulation of naive and memory CD8 T cells by DNA vaccination: a dendritic cell‐dependent process
Author(s) -
Bedoui Sammy,
Davey Gayle M,
Lew Andrew M,
Heath William R
Publication year - 2009
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.2008.105
Subject(s) - priming (agriculture) , antigen , immunology , biology , cytotoxic t cell , dna vaccination , cross presentation , t cell , cd8 , immunization , antigen presenting cell , immune system , microbiology and biotechnology , in vitro , genetics , botany , germination
CD8 T‐cell priming following DNA vaccination has been shown to confer protection against infections and tumors. These vaccines, however, have been disappointing in their ability to boost memory responses in prime‐boost settings. We recently found that migratory dendritic cell (DC) subsets inefficiently stimulate memory CD8 T cells, raising the possibility that the poor boosting capacity of DNA encoded antigens might relate to their presentation by subsets of DCs that are only capable of efficiently stimulating naive T cells. Here, we show that DCs are required for T‐cell priming in vivo following intradermal immunization with DNA‐encoded antigens and that epidermal Langerhans cells are relatively unimportant. We then provide evidence that naive and memory CD8 T cells respond equally to DNA‐encoded antigen. These findings show that immunization to DNA‐encoded antigens is strongly DC‐dependent and that the failure to boost memory T‐cell immunity efficiently is not a consequence of a differential capacity of this form of antigen to stimulate naive or memory T cells.