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cDNA cloning of porcine interleukin‐2 receptor‐α gene
Author(s) -
Kokuho Takehiro,
Uchimura Akihiko,
Inumaru Shigeki
Publication year - 1997
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1997.81
Subject(s) - cloning (programming) , complementary dna , interleukin 1 receptor , molecular cloning , biology , gene , microbiology and biotechnology , interleukin 1 receptor, type i , interleukin 12 receptor, beta 1 subunit , genetics , interleukin 21 receptor , interleukin , cytokine , computer science , programming language
Porcine interIeukin‐2 receptor‐a subunit (IL‐2Rα) cDNA was cloned from the cDNA library of Con A‐stimulated PBMC. The coding sequence of porcine IL‐2Rα, including the signal peptide sequence, is 813 b.p. in length. The identities of the sequence when it was compared with ovine, murine, feline and human Sequences were 72.2, 62.4, 69.8 and 68.9% at nucleotide level and 58.9, 44.6, 54.6 and 55.6% at amino acid level, respectively. Then, the coding sequence of porcine IL‐2Ra was subcloned into the COS expression vector, pcDNA3.1/Zeo(+), and transfected into COS‐7 cells. The expressed protein was specifically reactive to the mAb, 231‐3B2, which seemed to be specific for porcine IL‐2Rα. This result reciprocally confirmed that the mAb, 231‐3B2, recognizes porcine IL‐2Rα on a molecular basis.