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Primary cytotoxicity of SEB‐activated human PBMC and separated CD8 + and CD4 + T lymphocytes elicited by two different stimulation protocols
Author(s) -
SEPRÉNYI GYÖRGY,
ABE JUN,
KOHSAKA TAKAO
Publication year - 1996
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1996.80
Subject(s) - cytotoxicity , cd8 , peripheral blood mononuclear cell , microbiology and biotechnology , cytotoxic t cell , chemistry , immunology , biology , antigen , in vitro , biochemistry
Summary. The primary cytotoxicity of Staphylococcal enterotoxin B (SEB)‐stimulated PBMC and separated CD8 + and CD4 + T cells against Burkitt's lymphoma target cells has been characterized by applying two stimulation protocols. In the bulk protocol, the PBMC were stimulated with 100 ng/mL SEB for 3 days before separation to CD4 + and CD8 + T subsets. In the direct protocol, the separated CD4 + and CD8 + T cells were stimulated with 100 ng SEB preabsorbed to mitomycin C (MMC)‐treated APC. Comparison of the results of the two different protocols revealed the following differences: (i) PBMC in the direct protocol provided greater cytolytic activity than in the bulk protocol; and (ii) the CD4 + T cells acquired cytotoxicity only in the direct protocol. Unexpectedly, the superantigen‐dependent cellular cytotoxicity (SDCC) of SEB‐stimulated cells was not dominant compared with the basal cytotoxicity. The classical NK target, K‐562 was also sensitive to SEB‐augmented cytotoxicity. The parallel stimulation with IL‐2 and SEB caused a similar extent of cytotoxicity enhancement against both types of target cells. However, the cyclosporin A (CSA) inhibited only the SEB‐induced cytotoxicity. The results suggest that SEB‐induced PBMC acquire mainly a LAK‐like cytotoxicity, as a consequence of newly produced lymphokines. This observation might propose a different approach in pathological studies.