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Manipulation of the helper T cell response to influence antigenic competition occurring with a multivalent vaccine
Author(s) -
HUNT JACQUELINE D,
BROWN LORENA E,
WOOD PAUL R,
STEWART DAVID J,
JACKSON DAVID C
Publication year - 1996
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1996.11
Subject(s) - heterologous , priming (agriculture) , pilus , antigen , biology , antibody , population , microbiology and biotechnology , cell , t cell , immunology , immune system , biochemistry , virulence , botany , germination , gene , demography , sociology
Summary The reduction in antibody observed following inoculation with multiple heterologous Dichelobacter nodosus pili antigens is thought to be due to competition between antigen‐specific B cells for a limited amount of T cell help. We demonstrate here that this competition is not further influenced by the expansion of cross‐reactive antibody secreting cells at the expense of serogroup specific antibody secreting cells. The T cell determinants of pili recognized by sheep and BALB/c mice have been defined using 15 residue peptides. These T cell determinants include cross‐reactive determinants in the conserved amino terminal region of the antigen. Here we investigate the effect of expanding the pili‐specific T cell population by priming with pili derived T cell determinants. It was not possible to increase the antibody elicited in response to the multivalent vaccine by priming mice with either a synthetic peptide spanning a T cell determinant or with reduced and alkylated or heterologous serogroups of pili 4 weeks before inoculation with the multivalent vaccine. A strategy designed to increase the T cell population by inoculating animals with pili covalently coupled to an extrinsic T cell determinant was pursued.