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cDNA cloning of porcine granulocyte‐macrophage colony‐stimulating factor
Author(s) -
INUMARU S,
TAKAMATSU H
Publication year - 1995
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1995.74
Subject(s) - complementary dna , microbiology and biotechnology , cloning (programming) , signal peptide , biology , peptide sequence , coding region , nucleic acid sequence , granulocyte macrophage colony stimulating factor , molecular cloning , amino acid , gene , biochemistry , in vitro , computer science , programming language
Summary Porcine granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) cDNA was cloned by using polymerase chain reaction (PCR) and sequenced. A coding sequence of the porcine GM‐CSF precursor protein, including the signal peptide sequence and stop codon, is 435 bp in length. The identities of the porcine GM‐CSF coding sequence when compared to ovine, bovine, human and murine sequences were 89, 86, 83 and 70% at the nucleotide level, and 80, 74, 73 and 56% at the amino acid level. The hydrophobicity profiles, putative glycosylation sites and positions of cysteine residues were highly conserved in porcine, ovine, bovine and human GM‐CSF but not murine. This is the first report of the porcine GM‐CSF cDNA cloning and sequence.