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Prolactin and insulin regulate the release of IL‐1‐α and IFN‐γ from murine splenocytes activated with porins or LPS of Salmonella typhimurium
Author(s) -
VITIELLO MARIATERESA,
SCARFOGLIERO PASQUALE,
GALDIERO MARILENA,
GORGA FERNANDA,
SOMMESE LINDA
Publication year - 1995
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1995.70
Subject(s) - splenocyte , lipopolysaccharide , prolactin , salmonella , cytokine , insulin , in vitro , chemistry , endocrinology , medicine , biology , microbiology and biotechnology , immunology , biochemistry , bacteria , hormone , genetics
Summary Murine splenocytes treated with prolactin (PRL) or insulin were stimulated in vitro with porins or lipopolysaccharide (LPS) of Salmonella typhimurium. It was seen that PRL inhibits the release of IFN‐γ from splenocytes treated with porins by about 20% while having no effect on the release of IL‐1‐α. Splenocytes stimulated with LPS, in the presence of PRL, had a reduced release of IFN‐γ (35%) and IL‐1‐α (70%). The porin‐stimulated splenocytes exhibited a remarkable increase in IL‐1‐α release (100%) and a diminished release of IFN‐γ (about 50%) in the presence of insulin. The splenocytes stimulated with LPS had a reduced release of IL‐1‐α (75%) and IFN‐γ (about 50%) when insulin was added. The data suggest that the classical endocrine system participates in a bioregulatory feedback loop that may prevent unwanted toxicity from cytokine excess. However, some bacterial products sometimes enormously unbalance this regulatory network.