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Histopathology of the lung following intratracheal challenge with live Pseudomonas aeruginosa in intestinally immunized rats
Author(s) -
WILSON NR,
DUNKLEY ML,
BURET A,
YOUNG B,
CRIPPS AW
Publication year - 1995
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1995.68
Subject(s) - pseudomonas aeruginosa , lung , histopathology , immune system , mononuclear cell infiltration , infiltration (hvac) , pathology , inflammation , cellular infiltration , histology , biology , microbiology and biotechnology , immunology , medicine , bacteria , genetics , physics , thermodynamics
Summary This paper examines the histology of rat lungs following intestinal immunization with killed mucoid Pseudomonas aerugtnosa and subsequent pulmonary challenge with live P. aeruginosa. The lungs of non‐immune challenged rats developed a confluent haemorrhagic pneumonitis with degeneration and sloughing of the mucosa of the airways; perivascular infiltration with mononuclear cells was apparent 1–2 h post‐challenge; some neutrophils were present by 2 h post‐challenge; by 12h post‐challenge oedema and intra‐alveolar haemorrhage were prominent and Gram‐negative organisms were seen in large quantities. In contrast, immunized challenged animals showed a pronounced neutrophilic response 1–2 h post‐challenge; by 12 h post‐challenge patchy abscesses were apparent with resolving inflammation and no organisms visible. The findings suggest that intestinal immunization prevents the development of fatal P. aeruginosa infections in the lung by accelerating the recruitment of polymorphonuclear neutrophils