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The 65 kDa protein of Mycobacterium habana and its putative role in immunity against experimental tuberculosis
Author(s) -
SINGH NB,
SRIVASTAVA KANCHAN,
MALAVIYA B,
KANDPAL HEMA,
SRIVASTAVA ANIL,
GUPTA HP
Publication year - 1995
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1995.57
Subject(s) - mycobacterium tuberculosis , antigen , biology , heterologous , microbiology and biotechnology , immune system , mycobacterium leprae , mycobacterium , protein subunit , tuberculosis , virology , immunity , immunology , bacteria , gene , medicine , biochemistry , leprosy , genetics , pathology
Summary Mycobacteria including Mycobacterium tuberculosis and Mycobacterium leprae possess multiple antigens some of which inhibit other anti‐mycobacterial immune responses. Whole cell vaccines are not free from these suppressive molecules and may adversely affect the immunogenic response(s). Purified protein components having only immunogenic properties should prove to be superior vaccine(s). Mycobacterium habana , a candidate vaccine for mycobacterial infections has been dissected for analysing its antigenic myriad. A 65 kDa protein of this mycobacterium has been isolated and characterized for its protective and cell mediated immune responses. The protein was isolated in pure form using an isotachophoresis (SDS–PAGE filtration) technique and identified with low molecular weight markers along with mAb using the immunoblot technique. Mab IIH9 has identified a 65 kDa protein in M. habana. This protein has been found to be immunoprotective in mice against M. tuberculosis H 37 Rv infection. It generates high levels of DTH responses in mice against M. tuberculosis and M. leprae antigens and inhibits migration of sensitized cells under the antigenic influence of homologous and heterologous origin. Possibilities of developing this protein as a subunit vaccine are discussed in this report.