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Feedback suppression of B cell colony formation by supernatants of B colony cells: Role of immunoglobulin
Author(s) -
FERNANDEZ L. A.,
MACSWEEN J. M.,
FALLOWS G.,
ROBSON D. A.
Publication year - 1995
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1995.25
Subject(s) - b cell , biology , antibody , naive b cell , microbiology and biotechnology , cell , t cell , immunology , b 1 cell , immune system , antigen presenting cell , biochemistry
Summary We have reported previously that CD5 + B cells from mature B cell colonies provide a negative feedback signal to the growth of autologous B cell colonies. Now we have observed that supernatants from mature B cell colonies also provide a negative feedback signal to the growth of autologous B cell colonies. We investigated the mechanism of this effect by growing B cell colonies physically separated by a 0.45 μm filter from T cells in millicell‐CM chambers. Addition of colony supernatants to the T cell compartment reduced the number of B cell colonies by 28±6%. Colony numbers were reduced by 11±2 and 17±5% when the supernatants were added to the B cell or to both compartments, respectively. Pulsing T cells with the B cell colony supernatants before adding them to the colonies also decreased colony numbers by 33 ± 13%. The addition of exogenous Ig classes and IgG subclasses to B cells decreased B cell colony numbers, although the effect was variable. In the presence of T cells, IgG had the greatest suppressive activity and the subclass IgG4 was most suppressive. In the absence of T cells, high concentrations of IgG almost abolished B cell colony formation. We conclude that these supernatants provide a negative feedback signal either directly to B cells, or via T cells which may be mediated at least in part by Ig.