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Influence of compounds affecting synthesis, modification and transport of proteins on the expression and release of interleukin‐2 receptor
Author(s) -
KARLSSON HÅKAN,
NÄSSBERGER LENNART
Publication year - 1995
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1995.13
Subject(s) - endoplasmic reticulum , microbiology and biotechnology , intracellular , proteases , golgi apparatus , receptor , transmembrane protein , glycosylation , chemistry , biophysics , biology , biochemistry , enzyme
Summary One of the most central events during lymphocyte activation is the synthesis and release of IL‐2. IL‐2 induces the synthesis and expression of the IL‐2 receptor α‐chain (IL‐2R) on the lymphocyte as well as the release of a truncated form of IL‐2R (sIL‐2R). The two proteins are identical except for the absence of the transmembrane and intracellular part on sIL‐2R. We have in an in vitro model investigated the influence of certain compounds, affecting different parts of the proliferative response, on the release and expression of IL‐2R. We found the generation of the two molecules to have different sensitivity to blocking of protein synthesis, glycosylation, microtubular assembly and proteolytic activity. However, blocking of intracellular transport from Golgi to the endoplasmic reticulum, disassembly of actin filaments and disturbances in the intracellular sodium/calcium balance had identical effects on expression and release of the respective IL‐2R. These findings indicate a more complex and specific mechanism behind the generation of sIL‐2R than simply by shedding through the action of proteases on the membrane‐bound IL‐2R.