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Antigen‐specific B cells in tissues after oral typhoid vaccination
Author(s) -
GIBSON C. E.,
PENTTILA I. A.,
LEONG A. SY.,
ZOLA H.,
LABROOY J. T.
Publication year - 1995
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1995.10
Subject(s) - antigen , lamina propria , immunology , immunofluorescence , typhoid fever , microfold cell , biology , ileum , vaccination , lymphocyte , medicine , pathology , virology , antibody , epithelium , endocrinology
Summary Six human subjects who were to receive elective bowel surgery for a variety of diseases were vaccinated with the oral typhoid vaccine, Ty21a. Intestinal tissue (ileum in two, large intestine in four) removed 7‐26 days after the first dose of vaccine was examined for the presence and distribution of antigen‐specific B cells. This was compared with intestinal tissue derived from two unvaccinated controls. A number of B cell differentiation antigens were also assessed on these cells by immunofluorescence using dual‐labelling. Antigen‐specific cells were found randomly distributed in the lamina propria of all the vaccinated subjects in low frequency (6 ± 0.5 to 37 ± 31 [mean ± s.e.m.] antigen specific cells/10 mm 2 of tissue). The lymphocyte differentiation antigens CD45RA, CD45RO. L‐selectin, CD‐1 la CD‐38, CD‐44 and VLA‐4 were all found on antigen‐specific cells, but no particular pattern was recognizable in this small series of six subjects with different disease processes affecting the intestine.