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Immunosuppression by lymphokine‐activated murine killer cell line with B‐lymphoblast‐lytic activity in vitro
Author(s) -
IKEMOTO M.,
SUZUKI H.,
SUGIYAMA E.,
YAMASHITA N.,
TUNRU I. S.,
MATSUI S.,
KOBAYASHI M.
Publication year - 1994
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1994.56
Subject(s) - lymphokine , lymphoblast , biology , microbiology and biotechnology , lytic cycle , cd8 , immune system , cd3 , in vitro , lymphokine activated killer cell , clone (java method) , t cell , cell culture , thymocyte , immunology , chemistry , interleukin 21 , biochemistry , virus , dna , genetics
Summary The in vitro immunosuppressive effect caused by a murine lymphokine‐activated killer cell line with B‐lymphoblast‐lytic activity was studied. The cloned cells (named BC‐1.10, phenotype Thy1.2 + LFA‐1 + , TCR‐αβ − , TCR‐γδ − , FcγRII − , CD2 − , CD3ε − , CD4 − , CD8 − and express mRNA of ζ chain) suppressed LPS‐induced Ig synthesis by B lymphoblasts previously stimulated with LPS. Phasecontrast microscopy indicated disappearance of B lymphoblasts at 24 h after the addition of BC‐1.10 cells. This suppressive effect was reduced when BC‐1.10 cells were pretreated with anti‐LFA‐1 mAb, which inhibits cytotoxicity of this clone. These data suggest that the immunosuppressive effect of BC‐1.10 is due to an elimination of B lymphoblasts, and that one of the physiological functions of lymphokine‐activated killer (LAK) cells, which are induced as a consequence of immune reactions, might be immunosuppression.