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Role of insulin‐like growth factor‐1 on the kinetics of human lymphocytes stimulation in serum‐free culture medium
Author(s) -
SCHILLACI ROXANA,
RIBAUDO CLAUDIA M.,
RONDI CRISTINA M.,
ROLDÁN ALICIA
Publication year - 1994
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1994.45
Subject(s) - growth factor , clone (java method) , endocrinology , medicine , stimulation , insulin , insulin like growth factor , dna synthesis , cell growth , kinetics , biology , growth hormone , in vivo , hormone , in vitro , chemistry , receptor , dna , biochemistry , genetics , physics , quantum mechanics
Summary In a serum‐free medium addition of insulin‐like growth factor‐1 (IGF‐1) consistently enhanced lymphocyte proliferation response to PHA in a dose‐dependent fashion. This effect was produced by an acceleration in the expression of clone expansion and not in the number of proliferating cells. This was documented by kinetics data obtained from the first proliferation round of PHA‐stimulated lymphocytes, in which addition of IGE‐1 reduced G 1 ‐phase length, without changing Go‐phase, S‐phase or cloned size. The data were confirmed in 10‐day culture of stimulated lymphocytes where IGF‐1 only accelerated cell proliferation without modifying the area enclosed by the proliferation curve. As IGF‐1 is under the control of growth hormone, our results suggest that some of the immunoregulation effects ascribed to growth hormone in vivo could be produced by IGF‐1.