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Isolation and characterization of a neutrophil chemotactic factor from Tritrichomonas foetus organisms
Author(s) -
OWHASHI M.,
TOMIYOSHI F.,
HAYASHI H.
Publication year - 1994
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1994.37
Subject(s) - antigenicity , tritrichomonas foetus , chemotaxis , biology , size exclusion chromatography , antigen , western blot , immune system , microbiology and biotechnology , epitope , fetus , biochemistry , immunology , enzyme , pregnancy , genetics , receptor , gene
Summary A neutrophil chemotactic factor (TfNCF) was isolated from the crude extract of Tritrichomonas foetus organisms by a combination of anion‐exchange chromatography on DE52 and gel filtration on Sephacryl S200. TfNCF showed homogenicity by both PAGE and SDS‐PAGE. The molecular weight of TfNCF was estimated to be 22 and 24 kDa, by Sephacryl S200 gel chromatography and by SDS‐PAGE under reducing conditions. Immunization of TfNCF caused almost complete protection against T. foetus infection in mice. Western blot analysis probed with anti‐TfNCF antibody showed that the epitopes on TfNCF were not commonly shared on the other components of T. foetus organisms nor other helminthous parasite‐derived components. Furthermore, pre‐incubation of neutrophils with antigens of other helminthous parasites or N ‐formylmethionyl‐leucyl‐phenylalanine did not affect the neutrophil chemotactic activity for TfNCF. These results suggest that TfNCF is a novel NCF consisting of unique epitopes for both antigenicity and neutrophil chemotactic activity. The role of NCF in the initiation of the immune response is discussed.