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Heterogeneity of human anti‐Golgi auto‐antibodies: Reactivity with components from 35 to 260 kDa
Author(s) -
KOOY JACOBA,
TOH BANHOCK,
GLEESON PAUL A.
Publication year - 1994
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1994.19
Subject(s) - golgi apparatus , antibody , reactivity (psychology) , chemistry , biology , microbiology and biotechnology , immunology , medicine , endoplasmic reticulum , pathology , alternative medicine
Summary The Golgi auto‐antigens recognized by five human autoimmune sera were characterized with anti‐Golgi auto‐antibodies. The five sera showed strong anti‐Golgi reactivity, together with weak anti‐nuclear reactivity, as assessed by indirect immunofluorescence using the human HEp2 epithelial cell line. The Golgi auto‐antigens recognized by the autoimmune sera were identified by immunoprecipitation and immunoblotting of post‐nuclear supernatants. These sera reacted with at least 14 components of molecular mass ( M r ) 35–260 kDa; three components were detected only by immunoprecipitation, six components detected only by immunoblotting and five components appear to be detected by both techniques. We have previously shown that a patient with Sjögren's syndrome has auto‐antibodies specific for a 230 kDa Golgi auto‐antigens; a bacterial fusion protein containing auto‐epitope(s) of this Golgi auto‐antigen is not recognized by the other four autoimmune sera. Taken together, this study shows that the five anti‐Golgi autoimmune sera recognize distinct sets of auto‐antigens which include both conformational and/or sequential epitopes.