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Cyclic regulation of B220 antigen expression in immature B cell lines
Author(s) -
OKA Y.,
SHIMIZU Y.,
TSUKADA S.,
SUGIYAMA H.
Publication year - 1994
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1994.11
Subject(s) - antigen , cell culture , microbiology and biotechnology , biology , population , antibody , chemistry , immunology , medicine , genetics , environmental health
Summary Three independent immature B cell lines transformed with temperature‐sensitive mutants of Abelson murine leukaemia virus (A‐MuLV) persistently expressed B22O antigen detected by both of the anti‐B220 antibodies RA3‐6B2 and 14.8 during culture at the permissive temperature (35°C). RA3‐6B2 recognizes the B220 epitope and that 14.8 is a CD45RA antibody. However, when the culture temperature was shifted up to the non‐permissive temperature (39°C) and the culture was continued for 2–3 weeks, a population of the cells (RA3‐6B2 − 14.8 + cells) that lost RA3‐6B2 antigen appeared in all three cell lines. From these populations, RA3‐6B2 − 14.8 + subclones were independently isolated by limiting dilution and the phenotype was stable during culture at the permissive temperature. When the culture temperature of the RA3‐6B2 − 14.8 + subbclones was shifted up to the non‐permissive temperature and the culture was continued for 2–3 weeks, RA3‐6B2 antigen was re‐expressed in a subpopulation of the RA3‐6B2 − 14.8 + cells. These results demonstrated cyclic regulation of B220 antigen expression during proliferation and/or differentiation of immature B cell lines.