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Production of interleukin‐lα and interleukin‐2 by separate, phenotypically different leukaemia and T cell lymphotropic virus‐1‐transformed T cell clones
Author(s) -
HOLÁŇ V.,
MINOWADA J.
Publication year - 1993
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1993.56
Subject(s) - interleukin 2 , biology , virology , t cell , interleukin , virus , microbiology and biotechnology , immunology , cytokine , immune system
Summary The relationship between interleukin‐1 (IL‐1) and interleukin‐2 (IL‐2) production and immunophenotype marker profiles was studied in a panel of 29 leukaemia and human T cell lymphotropic virus‐l (HTLV‐1)‐transformed T cell lines. Culture supernatants from six of the 29 T cell lines tested increased IL‐2 production by the MOLT‐16 cell line in a manner similar to that of rIL‐1α or rIL‐1β. The enhancing activity in the cell culture supernatants was inhibited by antibody against IL‐1α. Anti‐IL‐1β antibody bad no inhibitory effect. All the cell lines producing IL‐1α had characteristics of activated mature T cells. They were terminal deoxyribonucleotidyl transferase (TdT) − , CD4 + , CD8 − , HLA‐DR + and all were strongly positive for IL‐2Rα (Tac antigen) expression. However, none of the IL‐1α producing cell lines secreted detectable IL‐2. A significant quantity of IL‐2 was found, after stimulation with phytohaemagglutinin, in supernatants from nine of the 29 cell lines tested. The majority of IL‐2 producing cell lines originated from less mature, non‐activated T cells, as they were characterized by the expression of TdT, lack of HLA‐DR antigens and > 50% had no detectable IL‐2Rα. The results thus show that separate, phenotypically different leukaemia and HTLV‐1‐transformed T cell clones produce IL‐1α and lL‐2. While IL‐1α production is exclusively confined to the cell lines having the phenotype of activated mature T cells, the IL‐2 producing cell lines have much more diverse origins in terms of their stage of maturation.