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Down‐regulation of E1a expression by E3 gene products in group C adenoviruses
Author(s) -
ZHANG XIAOLIU,
MÜLLBACHER ARNO,
BRAITHWAITE ANTONY W.
Publication year - 1992
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1992.10
Subject(s) - biology , transcription (linguistics) , mutant , microbiology and biotechnology , transfection , plasmid , gene , messenger rna , gene expression , regulation of gene expression , translation (biology) , virology , genetics , philosophy , linguistics
Summary Mutant group C adenoviruses defective in expression of the E3 transcription unit were found to overexpress E1a proteins relative to wild‐type adenoviruses. This result suggests that one or more proteins encoded in the E3 region (present in wild‐type viruses) down‐regulate E1a expression. This interpretation was confirmed by transfection experiments in which a plasmid expressing the E3 region reduced expression of E1a in 293 cells. Experiments to examine the molecular basis of this down‐regulation of E1a suggest that E3 protein products interfere with the translation of viral mRNA molecules.