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Conversion of the human blood group H antigen to A antigen in vitro
Author(s) -
YANG NAN,
BOETTCHER BARRY
Publication year - 1991
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1991.17
Subject(s) - antigen , in vitro , saliva , chemistry , antibody , sepharose , transferase , biochemistry , nitrocellulose , microbiology and biotechnology , h antigen , affinity chromatography , chromatography , biology , enzyme , immunology , membrane
Summary A‐transferase ( N ‐acetylgalactosaminyl transferase) was purified from human group A plasma using Sepharose 4B affinity ehromatography. Human anti‐A antibodies were purified from human serum by adsorption to an immunosorbent column and heat elution in order to detect the A antigen. Conditions appropriate for the development of the A antigen on O red cells were examined and several buffer systems were found to be equally effective. Expression of the developed A antigen was found to be similar to that on group A red cells, indicating that the system in vitro has similar activity to the system in vivo. The H antigen from human saliva was coupled to Sepharose 4B or adsorbed to a nitrocellulose membrane. The A antigen was able to be developed on these materials by the action of group A‐transferase. The procedures enabled the identification in vitro of sugar‐transferase activities which can be useful in studies within the A,B,H antigen system or other carbohydrate antigen system.