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The effect of glutamine on murine splenic leukocyte responses to T and B cell mitogens
Author(s) -
Griffiths M.,
Keast D.
Publication year - 1990
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1990.54
Subject(s) - phytohaemagglutinin , concanavalin a , glutamine , lipopolysaccharide , dna synthesis , mitogen activated protein kinase , b cell , biology , cell , microbiology and biotechnology , immunology , chemistry , lymphocyte , endocrinology , medicine , biochemistry , antibody , dna , in vitro , amino acid
Summary Murine splenic leukocytes cannot be stimulated to synthesize [ 3 H]‐DNA by various concentrations of either the T cell mitogens, Concanavalin‐A (Con‐A) or phytohaemagglutinin (PHA) or the B cell mitogen, lipopolysaccharide (LPS), unless glutamine is present in the culture medium. The optimum concentration of the T cell mitogens (Con‐A, PHA) remained constant for all levels of glutamine while that of the B cell mitogen (LPS) increased as the concentration of glutamine in the medium increased.