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Vaccinia virus‐mediated damage of murine ovaries and protection by virus‐expressed interleukin‐2
Author(s) -
Karupiah Gunasegaran,
Coupar Barbara,
Ramshaw Ian,
Boyle David,
Blanden Robert,
Andrew Marion
Publication year - 1990
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1990.44
Subject(s) - vaccinia , virus , biology , virology , titer , inoculation , orthopoxvirus , poxviridae , recombinant dna , ovary , immunology , endocrinology , biochemistry , gene
Summary Vaccinia virus was shown to replicate in the ovaries of normal inbred mice and cause sufficient damage to ovarian follicles to decrease fertility of the mice. The mouse‐adapted strain. VV‐WR, had a greater affinity for growth in ovaries than the vaccine strains, VV‐Elstree. VV‐NYBH or VV‐Copenhagen. Virus reached the ovaries after intravenous or intraperitoneal inoculation, but not after subcutaneous inoculation in the foot pad. Intcrleukin‐2 (IL‐2), when expressed by a recombinant vaccinia virus was able to decrease the titre of virus in the ovaries and prevent infertility. Both non‐specific (natural killer cells) and antigen‐specific mechanisms were active within the ovaries and may play a role in the IL‐2‐mediated clearance of vaccinia virus.