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The interleukin‐2 receptor on cord blood and nulliparous mononuclear cells is downregulated by maternal and cord blood serum
Author(s) -
Vanderbeeken Yves Edouard,
Duchateau Jean,
Boogaert L.,
Desseilles P.,
Collet Henry,
Lucas Adam
Publication year - 1990
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1990.34
Subject(s) - cord blood , peripheral blood mononuclear cell , endocrinology , downregulation and upregulation , medicine , receptor , pregnancy , interleukin 2 , stimulation , phytohaemagglutinin , cord , andrology , immunology , chemistry , biology , in vitro , biochemistry , genetics , gene , surgery
Summary The concentration of interleukin‐2 receptor(IL‐2R)on maternal, cord blood and unrelated nulliparous mononuclear cells has been studied after a previous phytohaemagglutinin (PHA) stimulation. Furthermore, the inhibitive action of maternal and cord blood serum on the IL‐2R expression of stimulated nulliparous and cord blood lymphocytes has been shown. A downregulation of the [ 3 H]‐thymidine uptake by these PHA treated cells previously incubated in maternal and cord blood serum has been observed. Retroplacental serum was the most inhibitive experimental medium. The IL‐2R modulation property of maternal serum has been studied during the pregnancy. Appearing quite early (in the sixth week), the maternal serum dependent inhibitive factor vanished, 2 to 3 weeks after delivery. After a study of the different serum components, it is suggested that the IL‐2R downregulating molecule is included in the maternal immunoglobulin (IgG) fraction. Further experiments suggest that the addition of recombinant IL‐2 during the action of low doses of maternal IgG allows a partial re‐expression of the IL‐2R. However, at physiological concentrations of IgG, the IL‐2R downregulation becomes irreversible.