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Functional studies of stable L cell transfectants expressing intercellular adhesion molecule 1 [ICAM‐1]
Author(s) -
Wawryk Stefan O,
Salvaris Evelyn,
Sandrin Mauro S,
Boyd Andrew W
Publication year - 1989
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1989.56
Subject(s) - transfection , cell adhesion molecule , intercellular adhesion molecule 1 , lymphoblast , microbiology and biotechnology , cell adhesion , icam 1 , intercellular adhesion molecule , biology , neural cell adhesion molecule , adhesion , cell culture , chemistry , cell , biochemistry , genetics , organic chemistry
Summary High molecular weight DNA isolated from human tonsil was transfected into mouse L cells to produce transfectants expressing the human intercellular adhesion molecule [ICAM‐1]. The transfected ICAM‐1 molecule was highly expressed on the cell membrane and our studies show that the transfected ICAM‐1 molecule was a fully functional adhesion protein. All leucocyte subtypes showed specific binding to the ICAM‐1 transfectants, their adhesion being inhibited by Fab 1 fragments of W‐CAM‐1 antibody and LFA‐1 MoAb (leucocyte function antigen). B cell lines(RAJI, Nalm1) showed the highest degree of ICAM‐1 mediated adhesion. Normal lymphoblasts showed comparable levels of binding whilst normal neutrophils (both resting and activated by (N‐formyl‐methionyl‐leucyl‐phenylalanine) fMLP) showed the least ICAM‐1‐mediated adhesion. Despite a significant level of adhesion to ICAM‐1 transfectants shown by T lymphoblasts generated in a two way MLR there was no evidence of cytolysis of ICAM‐1 transfectants. These studies demonstrate the potential of ICAM‐1 transfectants as tools for analysis of the role of ICAM‐1 in lymphoid adhesion.