Premium
Transfection of genes for human cell surface antigens identified by monoclonal antibodies
Author(s) -
Cole SR,
Kriek GW,
Hope RM,
Ashman LK
Publication year - 1989
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1989.54
Subject(s) - monoclonal antibody , transfection , antigen , gene , antibody , biology , microbiology and biotechnology , virology , immunology , genetics
Summary Six murine L cell lines expressing five different human cell surface antigens have been prepared by DNA‐mediated gene transfer. L tk − cells were transfected with calcium phosphate co‐precipitates of human genomic DNA and a plasmid containing the Herpes simplex thymidine kinase gene. After HAT selection, transfectants expressing specific cell surface antigens were identified by in situ immune resetting using monoclonal antibodies. In this way, transfected cell lines expressing the CD9 antigen, the CD31 antigen (two lines), a unique platelet antigen, an X‐linked antigen (R1), and a previously unreported monocyte antigen 11D1 were prepared. These cell lines have proved useful in the definitive assignment of monoclonal antibodies to specific CD groups. In addition, they provide a source of mRNA for use in expression cloning of the genes for these antigens.