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The murine cellular immune response to adenovirus type 5
Author(s) -
Müllbacher A,
Bellelt AJD,
Hla RT
Publication year - 1989
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1989.4
Subject(s) - immune system , biology , lytic cycle , major histocompatibility complex , effector , antigen , virology , cytolysis , t cell , adenoviridae , microbiology and biotechnology , immunology , virus , in vitro , recombinant dna , cytotoxic t cell , gene , genetics
Summary We have characterized the cellular immune response to human adenovirus 5 (Ad‐5) in mice, as a basis for future study of responses to foreign antigens in recombinant adenoviruses. Primary in vivo lytic effector cells contained both virus immune cytolytic T (Tc) cells and natural killer cells. The Tc effectors could be boosted in vitro to give a secondary Tc cell response. The Tc cell response to Ad‐5 was major histocompatibility complex (MHC) restricted, and in CBA/H (H‐2 k ) mice mapped to the K end of MHC. Kinetic experiments suggested the Tc cell response was directed against early rather than late viral proteins. Experiments with viral mutants showed that the main responses were to the E1A and E1B proteins, with some involvement of E2. Expression of E3 and E4 in infected targets was not required, in fact lysis of target cells infected by viruses with a deletion in E3 was augmented.