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Excretory‐secretory antigens from adult Nematospiroides dubius
Author(s) -
Monroy FG,
Adams JH,
East IJ,
Dobson C
Publication year - 1989
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1989.16
Subject(s) - antigenicity , glycoprotein , protease , antigen , gel electrophoresis , excretory system , biochemistry , biology , in vitro , polyacrylamide gel electrophoresis , microbiology and biotechnology , saliva , blot , staining , antibody , enzyme , chemistry , immunology , endocrinology , gene , genetics
Summary Adult Nematospiroides dubius excretory‐secretory (ES) products were collected from worms cultured in vitro , radiolabelled and separated by sodium dodecyl polyacrylamide gel electrophoresis (SDS‐PAGE). The components were characterized and assessed for molecular weight (MW) after autoradiography and gel staining, for antigenicity in immunoblots, for sensitivity to protease enzymes, and for carbohydrate content. ES contained at least 18 denatured components from MW <20 000 to >200 000. At least one of the surface proteins of adults was found with the ES antigens recovered when adults were cultured in vitro . Molecules with MW 200 000, 78 000 and 60 000 were glycoproteins and reacted with immune mouse serum in Western blots. The dominant ES, MW 60 000 component stained with periodic acid‐Schiff (PAS), bound lectins with affinity for D‐mannose, and was resistant to peptic and tryptic but not V8 protease digestion.