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A collagen matrix microassay for use in tumour‐stromal cell co‐cultures
Author(s) -
Agrez MV
Publication year - 1989
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1989.14
Subject(s) - stromal cell , cell culture , fibroblast , matrix (chemical analysis) , in vitro , cell , chemistry , microbiology and biotechnology , biology , cancer research , biochemistry , chromatography , genetics
Summary A collagen matrix microassay technique is described in which separation of collagen layers permits independent assessment of the proliferative capacity of each of two discrete cell populations, The two cell types used in this study were an established colon cancer cell line and a normal colon fibroblast cell line cultured under serum‐free conditions. The implications of this in vitro technique for studies of tumour‐host cell interactions are discussed.