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A portion of the Pf155/RESA antigen of Plasmodium falciparum is accessible on the surface of infected erythrocytes
Author(s) -
Saul Allan,
Lee Maloy W,
Rock Edwin P,
Howard Russell J
Publication year - 1988
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1988.36
Subject(s) - antigen , antiserum , biology , antibody , plasmodium falciparum , monoclonal antibody , microbiology and biotechnology , immunoprecipitation , red blood cell , peptide , virology , biochemistry , immunology , malaria
Summary An investigation of antigens accessible to lactoperoxidase‐catalysed cell surface iodination on intact Plasmodium falciparum ‐infected red blood cells (RBC) has identified a 125 I‐labelled antigen with an apparent size of about 155 kD. This labelled protein was specifically immunoprecipitated by the following antibodies: a rabbit antiserum and a mouse monoclonal antibody raised against a synthetic peptide comprising the 3′,8‐mer repeat EENVEHDA of the Pf155/RESA protein; a rabbit antiserum raised against a synthetic octapeptide comprising two copies of the 3′,4‐mer repeat EENV of the Pf155/RESA protein; and rabbit antisera against another synthetic peptide C(MYSNNNVED) 2 . The last antibody shows a strong reaction in asexual blood stage parasites with the Pf155/RESA antigen. While this antigen has been described previously as a submembrane component of the outer membrane of infected RBC, this report shows that at least part of it is accessible to the surface of both ring and late trophozoite‐infected erythrocytes.