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Proliferative activity in the proximal and distal colon of the rat after fasting and refeeding
Author(s) -
Butler RN,
Lawson MJ,
Goland GJ,
Jarrett IG,
RobertsThomson IC
Publication year - 1988
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1988.24
Subject(s) - in vivo , proximal colon , in vitro , distal colon , medicine , endocrinology , bromodeoxyuridine , cell growth , thymidine , biology , colorectal cancer , biochemistry , microbiology and biotechnology , cancer
Summary Several methods were used to assess proliferation of coloncytes in the proximal and distal colon of the rat after fasting and refeeding. Those applied in vivo included metaphase arrest, labelling with bromodeoxyuridine and uptake of tritiated thymidine. The latter two techniques were also applied after isolation of colonocytes in vitro . Methods applied in vivo showed similar proliferation in the proximal and distal colon after fasting and enhanced proliferation in both regions after refeeding. However, the degree of enhancement was greater in the distal colon than in the proximal colon. In vitro , proliferation was enhanced in both the proximal and distal colon after refeeding but the degree of variability was greater than after assessment in vivo . Methods applied in vivo are preferred for assessment of proliferative activity in the rat colon, and major changes in proliferation in both proximal and distal colon can be induced by fasting‐refeeding.