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Intracellular pathway of interleukin‐2 receptors studied using immunogold electron microscopy
Author(s) -
McCrae Cameron,
Maxwell Lesley,
Ceredig Rhodri
Publication year - 1988
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1988.23
Subject(s) - immunogold labelling , intracellular , electron microscope , microbiology and biotechnology , biophysics , receptor , chemistry , biology , biochemistry , physics , optics
Summary The fate of internalized interleukin‐2 receptors (IL‐2R) on proliferating T cell blasts and Lyt‐2 − /L3T4 − thymocytes was studied by immunogold electron microscopy. Immediately following labeling. IL‐2R were found in coated pits and with lime there was sequential appearance of gold particles in smooth vesicles or endosomes, multivesicular bodies and lysosomes. Thus the IL‐2R appears to be a member of cell surface receptors internalized by endocytosis via coated pits.