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Auto‐anti‐idiotypic antibodies in mice hyperimmunized with a chemically induced bladder carcinoma
Author(s) -
Greer Judith M,
Halliday WJ
Publication year - 1988
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1988.21
Subject(s) - idiotopes , antibody , immunoglobulin idiotypes , allotype , monoclonal antibody , immunogenicity , idiotype , biology , microbiology and biotechnology , immunology , humoral immunity
Summary Purified immunoglobulin from the serum of mice hyperimmunized with syngeneic tumour cells was compared with a monoclonal anti‐idiotope antibody, obtained by immunizing mice with antibody to the same tumour. Both the immunoglobulin and the monoclonal antibody were specifically immunogenic in syngeneic (BALB/c) mice as tested by in vitro and in vivo assays of cell‐mediated immunity. In both cases the reactivity was absent in immunoglobulin heavy chain gene complex‐1 ‐(Igh) allotype congenic (CB.20) mice, indicating Igh‐restriction of immunogenicity. The active material in hyperimmune serum thus has the properties of auto‐anti‐idiotypic antibody, complementary to T cell idiotopes.