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Festschrift In vitro synthesis of IgE by human peripheral blood leucocytes: VI Seasonal variations in synthesis of rye pollen ( Lolium perenne ) specific IgE
Author(s) -
Turner KJ,
Siemensma NP,
Krska KD,
Cameron KJ
Publication year - 1988
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1988.16
Subject(s) - lolium perenne , pollen , immunoglobulin e , biology , in vitro , peripheral blood , immunology , botany , antibody , poaceae , biochemistry
Summary Fractionation by Percoll density cetrifugation of peripheral blood leucocyte cells, from atopic subjects with seasonal hay fever, unmasked IgE‐B cell populations whose individual capacities to synthesize IgE in vitro were obscured in cultures of unfractionated B cells. B cell cultures from all six subjects in the study released rye pollen‐specific IgE during the 6 days of culture, but actual synthesis was significant only in October, the pollen season. Synthesis in October occurred most frequently in cultures of mature, low density B cells, which generally responded to the addition of autologous T cells with enhanced synthesis (T‐help). T‐help was also found for high density B ceils in the mid‐winter (July) cultures. Total IgE synthesis in vitro demonstrated a less seasonal relationship, although it tended to be maximal for low density B cell cultures in October and for high density B cells in May. All B cell cultures contained preformed total and rye‐specific IgE antibody which persisted throughout the pre‐ and post‐pollen seasons, particularly in the low density B cell fractions, even in the absence of de novo synthesis. Moreover, the intracellular levels of rye pollen‐specific IgE antibody were often higher in the winter than in the peak of the pollen season. The relevance of this preformed IgE remains to be established.