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Effect of dextran sulphate on IgG subclass of antibody in efferent popliteal lymph of sheep
Author(s) -
Kerlin RL,
Watson DL
Publication year - 1987
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1987.47
Subject(s) - lymph , antibody , lymphatic system , immunology , antigen , adjuvant , staphylococcus aureus , lymph node , immunoglobulin g , chemistry , hapten , immunofluorescence , subclass , microbiology and biotechnology , medicine , pathology , biology , bacteria , genetics
Summary IgG 1 and lgG 2 participation in anti‐hapten, anti‐carrier and immunoglobulin‐containing cell (Ig‐cc) responses was studied in sheep immunised with killed Staphylococcus aureus ‐Dinitrophenyl (DNP) conjugates. The antigen was given with or without the polyanionic adjuvant dextran sulphate (DXS). Animals were immunised intracutaneously on the lateral hock of one hind leg with 1 × 10 9 S. aureus ‐DNP with or without 20 mg DXS. Six weeks later, primed sheep underwent surgery to cannulate an efferent popliteal lymphatic vessel in the immunised leg. Forty‐eight hours after surgery, each sheep was given a further intracutaneous injection of 1 × 10 9 S. aureus ‐DNP with or without 20 mg DXS adjacent to the primary injection site. IgG 1 ‐cc and IgG 2 ‐cc in lymph were counted using indirect immunofluorescence. Total IgG 1 and IgG 2 anti‐staphylococcal and anti‐DNP antibody output in lymph was determined using enzyme‐linked immunosorbent assays (ELISA's). DXS markedly increased antibody responses to S. aureus ‐DNP in the popliteal lymph node of sheep and was shown to strongly enhance immunological memory. The ratios of IgG 2 ‐cc:IgG 1 ‐cc and lgG 2 :IgG 1 anti‐staphylococcal antibody in lymph‐draining popliteal lymph nodes of sheep stimulated with DXS and S. aureus ‐DNP was significantly greater than the same ratios in animals given antigen alone. The IgG subclass‐specific immunomodulatory effects of DXS were exerted whether the adjuvant was given with primary and/or secondary inoculations. There was no difference in the ratio of IgG 2 :IgG 1 anti‐DNP antibody in lymph from animals given S. aureus ‐DNP with and without DXS.