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Further studies on the heterogeneity of antigens recognised by CD‐1 monoclonal antibodies: distribution of epitopes and analysis of serological binding patterns
Author(s) -
Favaloro EJ,
Bradstock KF,
Grimsley P,
Henniker A,
Kamath S
Publication year - 1987
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1038/icb.1987.46
Subject(s) - monoclonal antibody , epitope , antigen , immunofluorescence , microbiology and biotechnology , serology , biology , antibody , monoclonal , virology , immunology
Summary The binding patterns of 28 monoclonal antibodies (MAB) recognizing antigens belonging to Cluster of Differentiation One (CD‐1) were analyzed in order to investigate heterogeneity within this cluster. Competitive binding assays using radiolabelled MAB provided detailed information on CD‐1 antigenic heterogeneity, and demonstrated that at least six epitopic regions are recognised as CD‐1 MAB. Further studies, based on single cell suspension immunofluorescence assays (using thymocytes and subclones on the cell line Molt‐4), suggested that the majority of MAB studied could be serologically separated into three groups. In view of the most recent information suggesting that CD‐1 MAB recognize at least three different early T‐cell differentiation molecules, our results indicate that there are three or more distinct epitopes on the ‘gp49’ (HTA‐1/CD‐1a) molecule, two on the ‘gp45’ (HTA‐3/CD‐1b) molecule and one on ‘gp43’ (HTA‐2/CD‐1c).