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PURIFICATION AND CHARACTERIZATION OF TOXOPLASMA GONDII TACHYZOITE DNA
Author(s) -
Johnsont Alan M,
Dubey JP,
Dame John B
Publication year - 1986
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1986.37
Subject(s) - toxoplasma gondii , isopycnic , dna , strain (injury) , biology , microbiology and biotechnology , ultracentrifuge , chemistry , biochemistry , centrifugation , genetics , antibody , anatomy
Summary Toxoplasma gondii DNA from RH strain tachyzoites was purified from contaminating murine DNA by isopycnic density gradient ultracentrifugation in CsCI containing the fluorescent dye Hoechst 33258. Quantitative hybridization studies with DNA from uninfected mice and DNA extracted from oocysts of the ME49 strain of T. gondii confirmed that RH strain DNA could be obtained at least 90% pure with a high recovery. Preliminary characterization studies on such purified DNA showed that it had a G + C content of 50% and was not significantly methylated.