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GENETIC ANALYSIS OF KUNJIN VIRUS ISOLATES USING HAE III AND TAQ I RESTRICTION DIGESTS OF SINGLE‐STRANDED cDNA TO VIRION RNA
Author(s) -
Lobigs M,
Weir RC,
Dalgarno L
Publication year - 1986
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1986.20
Subject(s) - haeiii , biology , virology , genetics , taqi , complementary dna , restriction enzyme , enzootic , restriction fragment length polymorphism , molecular epidemiology , genotype , virus , dna , gene
Summary The genotypic relatedness of 15 Kunjin (KUN) virus isolates from widely separated geographic regions in Australia was examined at the molecular level. Haelll and TaqI restriction digest profiles of cDNA transcribed from virion RNA revealed a close genetic similarity between all isolates. We estimate that the nucleotide sequence divergence between any pair of KUN isolates is probably less than 1%. We conclude that a single KUN genetic type has existed in enzootic and epizootic areas of virus activity over an extended time span. The epidemiological implications of these results are discussed.