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ISOENZYME STUDIES OF GIARDIA LAMBLIA ISOLATED FROM SYMPTOMATIC CASES
Author(s) -
Baveja Usha K,
Jyoti AS,
Kaur Manjit,
Agarwal DS,
Anand BS,
Nanda R
Publication year - 1986
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1986.13
Subject(s) - giardia lamblia , phosphoglucomutase , isozyme , biology , hexokinase , oxidoreductase , giardia , biochemistry , malate dehydrogenase , enzyme , glucose 6 phosphate isomerase , isomerase , microbiology and biotechnology , glycolysis
Summary Strains of Giardia lamblia were isolated from symptomatic cases of giardiasis and axenized in the laboratory. Electrophoretic mobility patterns of four enzymes, viz., EC 5.3.1.9 glucose phosphate isomerase (GPI); EC 1.1.1,4.0.L‐malate; NADP + Oxidoreductase (Oxaloacetate decarboxylating) (ME); EC 2.7.5.1 phosphoglucomutase (PGM); and EC 2.7.1.1 hexokinase (HK) of the lysates prepared from these isolates were studied using starch—gel. Based on differences in mobility patterns of PGM and HK, the four strains studied could be grouped into three different isoenzyme types (Zymodemes). ME mobility was identical in all the four strains. Some relative difference was seen in the mobility of GPI, though the pattern of mobility was similar in all the strains.