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RECONSTRUCTING NORMAL α‐CRYSTALLIN FROM THE MODIFIED CATARACTOUS PROTEIN
Author(s) -
Thomson John A,
Hum Tony P,
Augusteyn Robert C
Publication year - 1985
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1985.60
Subject(s) - crystallin , homogeneous , chemistry , biochemistry , molecule , biophysics , biology , organic chemistry , mathematics , combinatorics
Summary α‐Crystallin from human cataractous lenses is so extensively modified that it is unsuitable for structural studies. We have disassembled the protein and, from the remaining traces of unmodified polypeptides, reconstructed a more homogeneous and conformationally discrete molecule. We suggest that this approach may be particularly useful to studies of enzymology and molecular gerontology.