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ESTABLISHMENT AND PARTIAL CHARACTERISATION OF A HUMAN FIBROSARCOMA CELL LINE MR‐83
Author(s) -
Stuckly K,
Kiddle R,
Leversha M,
Ellis WM,
Ekert H
Publication year - 1985
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1985.48
Subject(s) - plating efficiency , fibrosarcoma , karyotype , cell culture , agar , epidermal growth factor , biology , chromosomal translocation , microbiology and biotechnology , chromosome , genetics , bacteria , gene
Summary We describe a mycoplasma‐free human fibrosarcoma cell line, MR‐83, which glows readily in liquid culture and as clones in semi‐solid agar with a plating efficiency of about 0.5%. It has a stable karyotype consisting of a modal number of 49‐51 chromosomes, with two translocations and a deletion. The cell line shows resistance to adriamycin in semi‐solid agar assay, and responds to Epidermal Growth Factor (EGF) by increased DNA synthesis, as measured by thymidine uptake.