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HUMAN TUMOUR CELL LINES ESTABLISHED USING CLONAL AGAR CULTURE
Author(s) -
Bertoncello I,
Bradley TR,
Webber LM,
Hodgson GS,
Campbell JJ
Publication year - 1985
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1985.27
Subject(s) - agar , clonogenic assay , clone (java method) , cell culture , biology , agar plate , karyotype , biopsy , microbiology and biotechnology , pathology , genetics , gene , bacteria , medicine , chromosome
Summary Four families of human tumour cell lines—one of uterine, and three of ovarian origin—were established at early passage level from primary biopsy specimens of terminal patients by the propagation of anchorage‐independent agar clones in liquid culture. All cell lines exhibited unique and stable characteristics and retained their ability to clone in agar. However, considerable heterogeneity was evident in clonogenic capacity, karyotype, and responsiveness to growth‐promoting substances even among progeny of single agar clones isolated from the one biopsy specimen. These cell lines will be made available for further study upon request.