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OPTIMISED GROWTH OF HUMAN EPIDERMAL CELLS IN VITRO WITHOUT THE USE OF A FEEDER LAYER OR COLLAGEN SUBSTRATE
Author(s) -
Thompson CH,
Rose BR,
Cossart YE
Publication year - 1985
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1985.16
Subject(s) - in vitro , substrate (aquarium) , layer (electronics) , microbiology and biotechnology , biophysics , biology , chemistry , materials science , nanotechnology , biochemistry , ecology
Summary A culture method has been developed which enables a reasonably large quantity of human epidermal cells to be grown in vitro without the use of irradiated 3T3 feeder layers or collagen substrates. These cultures become confluent within 2 or 3 weeks of initiation, and may be sub‐cultured several times over their 16‐week average lifespan provided that the calcium levels in the cultured medium are controlled. Some of the potential uses of these cultures are briefly discussed.