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PORTAL VEIN PERFUSION OF THE ISOLATED RAT LIVER: SOME MARKERS OF HYPERTHERMIC LIVER DAMAGE
Author(s) -
Wynne JM,
Mack S,
McRae D,
Pillay SP,
Potts J,
Boffinger C,
Cowley DM,
Egerton WS
Publication year - 1984
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1984.7
Subject(s) - hyperthermia , perfusion , liver injury , potassium , chemistry , medicine , pathology , biology , endocrinology , organic chemistry
Summary The cytotoxic effects of hyperthermia on tumours are readily measured. Parameters of injury to normal tissues are needed to evaluate therapeutic potential. Fifty‐five isolated rat fivers were perfused in vitro for 180 min in order to determine parameters of hyperthermic injury. During this period they were healed for 1 h at temperatures ranging from 37° to 45° and then assessed for evidence of hyperthermic injury. The most critical indicator of hyperthermic injury was sustained depression of bile production. Bile secretion decreased by 80% after heating at temperatures above 42°. Aspartate amino transferase (AST) release increased significantly at temperatures of 42° and above. Potassium and variable amounts of glucose were released into the medium during healing, reflecting temporary changes in metabolism at high temperatures.