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THE RESPIRATION AND ENERGY METABOLISM OF SHEEP LYMPHOCYTES
Author(s) -
Cheung K,
Morris B
Publication year - 1984
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1984.64
Subject(s) - respiration , metabolism , immune system , mole , lymph , energy metabolism , carbohydrate metabolism , antigen , biology , salmonella , medicine , chemistry , endocrinology , biochemistry , immunology , bacteria , botany , genetics , psychiatry
Summary The respiration characteristics and energy metabolism of sheep lymphocytes collected from the popliteal lymph before and after antigenic challenge with Salmonella organisms have been studied at various O 2 tensions. The O 2 consumption of sheep lymphocytes could be related to the PO 2 of the medium by the Michaelis‐Menten equation. In resting lymphocytes, Vmax (mean ± S.D.) was 1·70 ± 0·23 μmoles/h/2 × 10 8 cells), critical O 2 concentration ([O 2 ]crit) was 2·00 ± 0·20 μM and Km was 0·46 ± 0·11 μM. Significant chances occurred in the respiration characteristics of these cells following antigenic challenge of the nude. Both Vmax and Km reached maxima when the blast cell response in the lymph was at its peak. [O 2 ]crit, however, did not change significantly over an immune response. Lymphocytes collected before antigenic challenge had mean rates of glucose uptake of 0·97 ± 0·29 μmoles/h/2 × 10 8 cells and lactate production of 1·31 ± 0·37 μmoles/h/2 × 10 8 cells. During the height of immune responses to Salmonella , lymphocytes showed an enhanced glucose uptake, lactate production and C‐1‐ and C‐6‐labelled glucose metabolism. The rates of energy metabolism of sheep lymphocytes varied with changes in the PO 2 of the gas phase from 21% to 0% O 2 . The rates of glucose uptake and lactate production increased while the rates of 14 CO 2 production from C‐1‐ and C‐6‐labelled glucose decreased as the PO 2 decreased.