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MONOCLONAL ANTIBODIES SPECIFIC FOR BLOOD GROUPS A AND B
Author(s) -
Fletcher A,
Harbour C,
Zwart R
Publication year - 1984
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1984.40
Subject(s) - monoclonal antibody , antibody , immunology , medicine
Summary Monoclonal antibodies have been produced with specificity for human blood group A following the immunization of mice with group A 1 cells. The antibodies produced were of low titre and poor avidity and were therefore unsuitable as manual grouping reagents. However, in 6,524 automated tests on the Technicon BG15 monoclonal anti‐A performed, as well as the standard human anti‐A reagent. Monoclonal anti‐B antibodies were produced from a BALB/c mouse immunized with a combination of group B cells and B substance. One antibody, B/C9/F7, performed well in screening tests with avidity reactions <10 seconds with all B, A 1 B and A 2 B cells tested. This antibody performed with 100% accuracy in an automated test trial involving 1,018 samples. The suitability of these antibodies as blood grouping reagents is discussed.