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IMMUNOGENICITY OF RNA‐PROTEIN FRACTION ISOLATED FROM AXENIC ENTAMOEBA HISTOLYTICA (NIH:200)
Author(s) -
Sharma GL,
Naik SR,
Vinayak VK
Publication year - 1984
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1984.11
Subject(s) - immunogenicity , entamoeba histolytica , adjuvant , immunization , immune system , rna , axenic , biology , immunology , virology , precipitin , antibody , microbiology and biotechnology , antibody response , antigen , bacteria , biochemistry , genetics , gene
Summary The immunogenicity of Entamoeba histolytica RNA (EhRNA) was studied in guinea‐pigs. Animals immunized with a single dose of EhRNA and Freund's complete adjuvant (FCA) showed a high level of cell‐mediated immune response (CMIR) as assessed by percent leucocyte migration inhibition (74·8 ± 2·9) after 1 week of immunization. This response faded away gradually and by week 5 after immunization the percent leucocyte migration inhibition was 27·1 ± 8·27, which is quite close to control values. The immunogenic activity of EhRNA was found to be specific, since yeast RNA failed to elicit immunological responses, and was RNase‐sensitive. The CMIR could be maintained at its high level by giving boosters or multiple doses. Anti‐EhRNA antibodies (haemagglutinins or precipitins) could not be demonstrated at any stage.