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ADHERENCE OF MURINE PERIPHERAL BLOOD EOSINOPHILS AND NEUTROPHILS TO THE DIFFERENT PARASITIC STAGES OF NEMATOSPIROIDES DUBIUS
Author(s) -
Penttila IA,
Ey PL,
Jenkin CR
Publication year - 1983
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1983.58
Subject(s) - parasite hosting , antibody , biology , receptor , immunology , nematode , centrifugation , immune system , larva , antigen , differential centrifugation , egta , microbiology and biotechnology , biochemistry , medicine , calcium , ecology , world wide web , computer science
Summary Eosinophils and neutrophils, purified by density gradient centrifugation from the blood of infected mice resistant to reinfection, were tested for their ability to adhere to the different parasitic larval stages of the murine nematode parasite Nematospiroides dubius . Cells were tested for adherence to larvae which had been sensitised with immune mouse serum (IMS) or normal mouse serum (NMS) in the presence of Ca 2+ and Mg 2+ ions, EDTA, or EGTA. Differences were observed in the degree of cell adherence to the different stages of the parasite. However, the adherence of the two cell types to any given stage of the parasite was similar. Adherence to the sheathed infective third‐stage (L3) larvae, 96 h post‐infective larvae and to adult worms depended to a large degree on conditions suitable for complement activation ( viz . fresh serum and the presence of Ca 2+ and Mg 2+ ions). Complement was activated both via the alternative pathway by the parasite itself and via the classical pathway by parasite‐bound antibodies. In these conditions, cell adherence probably occurred predominantly through the interaction of leucocyte third component of complement (C3) receptors with parasite‐bound C3. In contrast, adherence of cells to exsheathed L3 and to the 48 h and 72 h post‐infective larval stages appeared to involve antibody/Fc receptor as well as C3/C3 receptor interaction. The data indicate that N. dubius may undergo a series of antigenic changes during its life cycle and that antibodies capable of mediating granulocyte attachment are elicited predominantly against the early tissue developmental forms of the parasite.