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RAT POST‐IMPLANTATION EMBRYO CULTURE USING HETEROLOGOUS SERUM
Author(s) -
Priscott PK
Publication year - 1983
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1983.5
Subject(s) - heterologous , embryo , biology , ascitic fluid , embryonic stem cell , embryo culture , andrology , embryogenesis , immunology , medicine , microbiology and biotechnology , biochemistry , ascites , gene
Summary In the present study sera from twelve species have been compared to rat serum and ascitic fluid for their ability to support the growth of post‐implantation rat embryos. No alternative to rat serum or rat ascitic fluid was found. The basis for this apparent species specificity was further analysed. A marked improvement in embryonic growth was seen when rat serum was added to the heterologous serum prior to culture, whereas replenishment of a heterologous serum several times during culture had no effect. The experiments suggested that heterologous sera were nutritionally inadequate but not simply in energy source. Possible mechanisms for the specificity arc discussed.