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LIMITATIONS OF STANDARD FILTRATION TECHNIQUES IN MEASURING SPECIFIC BINDING OF TRANSPORTED SUBSTRATES TO RENAL PLASMA MEMBRANES
Author(s) -
Miller John H,
Wesniewski Jerome W
Publication year - 1983
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1983.32
Subject(s) - membrane , filtration (mathematics) , chemistry , chromatography , renal cortex , kidney , biophysics , biochemistry , biology , endocrinology , mathematics , statistics
Summary Under equilibrium conditions, the specific binding of 14 C‐α‐methyl‐D‐glucoside and 14 C‐p‐aminohippurate to brushborder and basolateral membranes of rabbit kidney cortex could not be quantitated using standard filtration techniques. It was concluded that the low receptor affinities of these transported solutes and the masking of bound isotopic counts by vesicular uptake prevented accurate measurement of specific binding activities.