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QUANTITATION OF H‐2 ANTIGEN EXPRESSION ON HEPATOCYTES AND MACROPHAGES: EVIDENCE FOR AN INFLUENCE BY STRAIN BACKGROUND
Author(s) -
King Nick JC,
Parr EL
Publication year - 1982
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1982.67
Subject(s) - congenic , antigen , major histocompatibility complex , strain (injury) , microbiology and biotechnology , locus (genetics) , chemistry , biology , hepatocyte , gene , in vitro , biochemistry , immunology , anatomy
Summary The surface concentration of H‐2 complex antigens was measured by a quantitative immunoferritin labelling technique on hepatocytes and macrophages from five mouse strains with three haplotypes. The concentration of H‐2 molecules was similar on macrophages from three B10 congenic strains, and the same similarity was observed on hepatocytes from the three strains. With both hepatocytes and macrophages, however, the surface concentration of H‐2 d molecules on BALB/c cells was about twice that on B10.D2 cells, and that of H‐2 k molecules on C3H/HeJ cells was about twice what we observed on B10.BR/ SgSn cells. These measurements suggest a regulation of the surface concentration of H‐2 complex antigens by a locus outside the MHC.