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ALVEOLAR MACROPHAGES: FUNCTIONAL HETEROGENEITY WITHIN MACROPHAGE POPULATIONS FROM RAT LUNG
Author(s) -
Holt PG,
Warner LA,
Papadimitriou JM
Publication year - 1982
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1982.63
Subject(s) - alveolar macrophage , macrophage , lung , pulmonary alveolus , population , stimulation , avidity , pathology , immunology , chemistry , biology , medicine , endocrinology , antigen , biochemistry , in vitro , environmental health
Summary Alveolar microphages were obtained from the unstimulated lungs of rats by repeated endobronchial lavage and an interstitial macrophage population prepared by mincing the lungs subsequent to the lavage process. Considerable heterogeneity was observed within these macrophage populations with respect to cell size, surface morphology and cytochemistry. Functional studies, involving assessment of IgG‐Fc receptor density/avidity and the expression of cytostatic activity in cultures of mitogen‐stimulated lymphocytes, revealed comparable heterogeneity, and further indicated a considerably higher degree of apparent stimulation in the alveolar versus the interstitial macrophage population. Parallel assessment of the functional activity of blood monocytes, the immediate precursors of these latter macrophages, indicated a lower state of activation again. This suggests that the lung interstitium normally provides an intermediate environment between the blood and the alveolar spaces, wherein blood monocytes may undergo maturational changes before their efflux into the alveoli.